Exhaled nitric oxide analysis

INTRODUCTION — Nitric oxide (NO) is a molecular gas previously considered to have a health-related role only in the context of its formation from the combustion of fossil fuels and its contribution to air pollution. However, this view has been greatly modified since the discovery in 1987 that the free radical NO was the previously uncharacterized endothelial-derived relaxing factor. It has now become clear that NO plays an important role in most human organ systems.

Within the respiratory system, NO regulates vascular and bronchial tone (promoting dilation of both vessels and airway), helps to facilitate the coordinated beating of ciliated epithelial cells, and is an important neurotransmitter for non-adrenergic, non-cholinergic neurons which run in the bronchial wall [1-7]. This molecule can be detected in exhaled gas in concentrations, which vary in health and disease.

The biology of NO, techniques available for measuring this gas in exhaled breath, and potential uses of these measurements in clinical practice will be reviewed here. The therapeutic use of inhaled NO is discussed separately. (See "Inhaled nitric oxide in adults with pulmonary hypertension" and see "Novel therapies for the acute respiratory distress syndrome").

FORMATION OF NO — In biological systems, NO is formed by the action of one of the isoforms of the enzyme nitric oxide synthase (NOS). Three such isoforms have been identified and are termed type I or neuronal NOS (nNOS), type II or inducible NOS (iNOS), and type III or endothelial NOS (eNOS) (show table 1) [8,9]. Although these enzymes are distinct proteins encoded by genes on disparate chromosomes, all three catalyze the adduction of the guanidino nitrogen of the amino acid arginine to molecular oxygen, yielding NO and water (show figure 1).

Regulation of NOS — While the three isoforms of NOS catalyze the same reaction, regulation of the activity of these isozymes occurs through distinct processes. Both nNOS and eNOS are usually constitutively active and produce low amounts of NO, with output varying with changes in the intracellular calcium concentration. In contrast, iNOS binds calcium so avidly that its function is not influenced by calcium fluxes within the physiologic range. While not constitutively active in most settings, it has the capacity to generate large quantities of NO when transcriptionally upregulated by the inflammatory cytokines tumor necrosis factor-alpha (TNFa), interleukin 1-beta (IL-1B), and interferon-gamma (IFN-gamma) [8,9]. In vitro evidence suggests that this upregulation can be abolished by glucocorticoids [10].

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